Coding

Part:BBa_M34573:Design

Designed by: Nathan Kipniss, Shambhu Koirala, Tyler Wagner   Group: MIT BE 20.20 - S11   (2011-05-09)


Coding Sequence for Arsenic Transport 2, from Arabadopsis Thaliana


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 5116
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1681
    Illegal BglII site found at 3633
    Illegal BglII site found at 4753
    Illegal BamHI site found at 4285
    Illegal XhoI site found at 5245
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 4542
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4680
    Illegal BsaI.rc site found at 2213
    Illegal SapI.rc site found at 3034
    Illegal SapI.rc site found at 4067


Design Notes

Unwanted restriction sites had to be removed.


Source

http://www.ncbi.nlm.nih.gov/nuccore/NM_129020.3

References